Sentence examples for transformants on a from inspiring English sources

Through counter-selection against ectopic transformants identification of targeted mutants is facilitated by growing transformants on a medium supplemented with 5-fluoro-2′-deoxyuridine.

Three thousand variants were screened by growing the transformants on a 1 15 ratio of xylose (1 %) and glucose (15%%) with mineral medium using the 96-well plate format and Synergy MX (BioTek Instruments, Inc , USA reader.

For the assay using the KI-starch method it proved to be necessary to grow the Pichia transformants on plates composed of a layer without starch on top and a layer with it on the bottom.

(A) Growth of transformants on YPD medium.

To minimize positional effect of reporter gene expression, at least 1000 transformants on the membrane were collected to create a mixed population of transformants, which were cultured and assayed for the strength of GFP florescence.

We screened a total of 30,000 transformants on 3- N-morpholino propanesulfonic acid (MOPS)-XP plates after incubation for 2 days at 3- N-morpholino propanesulfonic

XAS transformants, on the other hand, were taller than non-transformed tobacco plants and had a more extensive root system (not shown in potted plants).

For this reason, we also generated a second insertional mutant collection in parallel by transforming N. gaditana cells by electroporation and selecting transformants on plates containing zeocin [ 35].

Interactions were assessed by plating several double transformants from "non-selective" media (media that allows for identification of the double transformants) on which growth is possible only if there exists an interaction between the test subjects.

As a result, total FAME productivity of the transformants on day 12 was similar to that of WT.

In a second approach, the phenotypic analysis of primary transformants on canavanine before recombination can be skipped.