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In contrast, the stigmas of SRK-SCR transformants of some other accessions, such as Col-0 and Rld, express transient SI: they exhibit a robust SI response only during a narrow window of stigma development (stage 13 and early stage 14), after which the ability of their stigmas to inhibit "self" pollen is weakened and they set seed (Nasrallah et al. 2002).
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Chen, H., Nelson, R. S. & Sherwood, J. L. Enhanced recovery of transformants of Agrobacterium tumefaciens after freeze-thaw transformation and drug selection.
Here, we report an efficient method to produce stable RNAi transformants of F. oxysporum using Agrobacterium-mediated transformation (AMT).
#18: the primary transformant of Pubi-EXG1 (Taichung 65), #21-3 and #27-4: the self-progenies of the primary transformants of Pubi-EXG1 (Taichung 65), v: the vector transformed control plant.
Escherichia coli λBL21DE3* transformants of pET24a-Cas9 were selected on LB plates containing 100 µg ml−1 kanamycin.
Transformants of pUbDetec16-GAP1 emitted fluorescence, while the pUbDetec16-GAP1K9R,K16R,K76R transformants did not.
Phenol-oxidizing activity was detected from transformants of the yeasts, indicating that the obtained cDNA encodes a laccase.
Revertants and partial transformants of rat fibroblasts infected with Fujinami sarcoma virus.
Arginine auxotrophs were found in one of 32 transformants of pyrG13 (targeting frequency of 3%) and in 29 of 31 transformants of MR12 (94%).
Samples T1-T3 are transformants of A. nidulans carrying the G. graminis lox gene, while M1-M3 are transformants of A. nidulans carrying the mutated G. graminis lox gene.
The best transformants of each of the gene combinations were chosen.
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