Arabidopsis transformants carrying either of the GmNPR1 genes showed induction of SAR in response to infection with the avirulent strain, Pst DC3000 carrying AvrRpt2.
However, analysis of transformants containing either β-glucuronidase (GUS) or luciferase (LUC) genes, with and without an intron revealed no detectable marker gene expression.
Selection of transformants carrying either pGBKT7-CdICE1/-21/-85/-121 outpGBKT7 was carried ont on SD/-Trp medium.
This strain allowed the selection of prototrophic transformants with either replicating or non-replicating plasmids containing the wild-type pyrF gene.
Using our standard Agrobacterium-mediated transformation protocol for potato [ 43], we failed to recover any transformants of potato cultivar Iwa with antisense constructs for either the GSL1 or GSL2 genes.
Here, we report an efficient method to produce stable RNAi transformants of F. oxysporum using Agrobacterium-mediated transformation (AMT).
#18: the primary transformant of Pubi-EXG1 (Taichung 65), #21-3 and #27-4: the self-progenies of the primary transformants of Pubi-EXG1 (Taichung 65), v: the vector transformed control plant.
Transformants of pUbDetec16-GAP1 emitted fluorescence, while the pUbDetec16-GAP1K9R,K16R,K76R transformants did not.
Arginine auxotrophs were found in one of 32 transformants of pyrG13 (targeting frequency of 3%) and in 29 of 31 transformants of MR12 (94%).
Samples T1-T3 are transformants of A. nidulans carrying the G. graminis lox gene, while M1-M3 are transformants of A. nidulans carrying the mutated G. graminis lox gene.
