Sentence examples for transformants of a from inspiring English sources

For the wild-type rescue construct we generated P-element transformants of a hybrid fusion gene that expresses the dCTCF cDNA under the control of the ubiquitously expressed hsp83 promoter [ 64, 65].

In the EcoRV digested genomic DNA of P. anserina wild-type strain s and in two secondary transformants of a deletion strain hybridised with a PaMth1 specific probe a 3.5 kbp band is only detected in the sample of the wild-type strain s and not in the deletion strain.

The presence in each transformant of a plasmid carrying mutated ssp4 sequences was demonstrated by PCR (Fig. 1A) and the presence of the desired ssp4 ORF mutation in the transformant was confirmed by sequencing (not shown).

The G54 strain has been shown to be a type 19F transformant of a serogroup 15 isolate (20 ).

The frequency of transformation was determined by calculating the number of transformants for a given amount of viable cells (5×108 bacteria) with 1 µg DNA of the suicide plasmid (pILL796).

These are dominant mutants because the phenotypes appeared in the primary transformants (T0) of a selfing plant [28].

To determine whether disruption of the NRPD1a gene could cause breakdown of SI in SRKb-SCRb transformants of accessions that express a developmentally stable SI response, an nrpd1a mutant in the C24 background (Zheng et al. 2008) was obtained and crossed to a C24[ SRKb-SCRb] plant.

Similar results were obtained in a cross between a Col-0 nrpd1a-8[ SRKb] homozygote and an SRKb-SCRb transformant of Sha, an accession that expresses developmentally-stable SI like C24.

A large number of transgenic wheat plants were generated and subjected to successive screenings and selection of FHB resistant plants starting with the primary transformants for a period of over four years.

Using an N-terminal SUMO tag, we initially obtained no transformants of the kinase domain in an expression strain, suggesting that the kinase was toxic.

On CMC plates, the pHX04 transformant demonstrated a higher endoglucanase activity than the transformants of pHX02.