Sentence examples for transformants obtained from from inspiring English sources
PCR-based replicon typing was applied to type the resistance plasmids carried by 26 Escherichia coli transconjugants or transformants obtained from epidemiologically unrelated clinical isolates of Enterobacteriaceae associated with community- or hospital-acquired infections in the United States or southern Europe Italy and GreeceEurope Italy and Greece
Five co-transformants obtained from two independent callus lines were identified in tissue culture by PCR using primers specific for the coding regions of the transgenes.
60 putative transformants were obtained on kanamycin containing plates, and 6 real transformants were obtained from these 60 putative transformants by spraying with glyphosate.
Fifteen and 23 transformants were obtained from transformation with the constructs.
The recipient genomic DNA was isolated from transformants obtained under the following co-cultivation conditions: the ratio of bacterial cells to target conidia was 5 × 103 or 1 × 104 on solid IM (Table 3, 48 h) and 50 in liquid IM (Table 5, 48 h).
From the transformants obtained, those that could not grow on plates containing galactose (for induction of αSyn expression) and 5-FOA (for loss of the cDNA plasmid) were selected for an αSyn toxicity test.
In this study, T-DNA insertion loci were recovered from L. maculans transformants obtained by ATMT for which germinating conidia (incubated for 48 hr) were used (Blaise et al. 2007).
Paromomycin-resistant transformants were obtained from all the co-bombardments, and, in all transformants, the presence of the aphVIII gene was verified by genomic PCR.
Transformation rates represent the number of transformants obtained per viable cell for 1 µg of DNA.
The presence of the respective construct in the transformants obtained was confirmed by PCR on DNA extracted from the different colonies using specific oligonucleotides for the specific inserts.
About 55 transformants were obtained from 106 protoplasts in a total volume of 150 μl containing 10 to 20 μg plasmid DNA.
