Suggestions(2)
Exact(3)
Therefore, it is reasonable to speculate that the uniform and dispersive mycelia of the TU6-vgb+ transformants might be the result of the expressed VHb, and its resulting improved oxygen transfer in submerged fermentation.
Genetic analysis indicated that the mutation of OsBRI1 (d61-1) [10] is epistatic to OsLIC (Figure 1H), which suggests that the increased leaf angle in OsLIC transformants might be due to activated BR synthesis.
The data allow just a qualitative estimation, because a) they were obtained under varying other conditions in the course of optimization, b) poor results disqualified a condition without rechecking, and c) some of the transformants might be false-positives as only a few were analyzed in detail.
Similar(57)
The low proportion of ∆-desaturated products (GLA and STA) found in the yeast transformant might be a result of a difference in codon usage between Pythium and S. cerevisiae.
Thus, the selected transformant expressing N355Q might be the result of the integration(s) at a locus (loci), leading to higher expression and/or of higher copy number than transformants expressing the wild-type pro-TGase.
Transformants with elevated laccase production might be generated by modifying the pGL promoter region and/or by screening among a larger number of co-transformants.
In this study, the whole-genome sequences of M184 and three second-generation transformants were analyzed to identify mutations that might be responsible for patAB overexpression.
However, preparation of fungal protoplasts was laborious, and heterokaryotic transformants might have genetic stability concerns [ 10, 11].
Frozen stocks of single transformants and pools of transformants were prepared by standard methods.
These transformants were named transformant cphA6803# 1and transformant cphA6803 2.
The plasmid from one transformant remains to be identified.
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