We believe that the synthesis of meso-2,3-butanediol in this transformant may be controlled by other rate-limiting factors.
For example, the conversion from acetoin to meso-2,3-butanediol also needs nicotinamide adenine dinucleotide (NADH) as the electro-donor [ 6, 18]; this NADH in the transformant may be the controlling factor, although the activity of meso-BDH is higher in the strain.
To avoid false positive transformants that may be generated from direct screening for glyphosate tolerance, T-DNA mediated transformants were first screened for kanamycin resistance, a marker carried from the vector pBI121.
The variation of transformant cell density may be derived from different loci and copy numbers of the integrated transgenic fragments [ 5, 26, 27].
Prey plasmids that can be recovered as stable transformants in E. coli may be retested by retransforming into yeast and rechecking the Leu+ and X-Gal+ phenotypes, along with controls that would show whether these phenotypes are specific for the bait interaction.
The consistent difference in Chl and Pchlide accumulation between the chlB_5 and chlB_6 mutants that are plastid transformants responsive to day length may be because of genetic differences between male (Tak-1) and female (Tak-2) lines in M. polymorpha.
However, we found that the RalAS194A mutant could not block cell aggregation induced by Aurora-A in the Ha- rasV12 transformants indicating that different signaling pathways may be transduced to control motility and aggregation of the different cells.
In addition, this system could also be utilized for temporary phytosensing without the need for deploying stable transformants, since levels of transient expression may be much higher than that of stable transgenic plants [ 16, 31], which is a major advantage for phytosensing.
It was found that up to 90% of clones in yeast homologous recombination libraries may be multiple vector transformants, that on average these clones bear four or more unique mutant genes, and that these multiple vector cells persist as a significant proportion of library populations for greater than 24 hours during liquid outgrowth.
