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In contrast, screening of 16 pools of bacterial API-expressing transformants led to the facile identification of API M358R and M358K as functional variants.

The constructs, pAKEnt and pAKFpvA, were mobilized in to Rhizobium sp. strains IC3123 and ST1 and expression of the corresponding gene in transformants led to utilization of the respective siderophores and stimulation in the growth of the transformants in the presence of respective siderophore producing strains in a co-inoculation study.

Nevertheless, greater carbon assimilation and electron transport rates (J and Jmax) for transformants led to greater yield increases than WT at elevated [CO2] compared to ambient grown plants.

After 10 14 days of incubation in the presence of antibiotic, re-greening of flasks indicated the initial presence and reproduction of at least one paromomycin-resistant P. morum transformant that led to a population of transformants.

Overexpression of S-adenosylmethionine decarboxylase in rodent fibroblasts led to aggressive transformants (Amdc-s cells) that had unforeseen high invasive capacity in nude mice, invading rapidly from the subcutaneous injection site into the peritoneal cavity and its organs.

First, the genetic transfer of metabolic mutations (pgkEP, pgm8, pgmIP or pykAJP, labeled by a genetic marker) into parental dnaE(Ts) strains led to Tr transformants.

After 9 12 days of incubation in the presence of antibiotic, re-greening of flasks indicated the initial presence and reproduction of at least one paromomycin-resistant E. elegans cell, which led to a population of transformants.

After another 9 16 days of incubation in the presence of the antibiotic, greening of a flask showed the initial presence of at least one paromomycin-resistant cell that led to a population of transformants.

Six and seven transformants were obtained receiving each construct; plasmid analyses led to the selection of four clones, ΔospC/ΔNt5/1, ΔospC/ΔNt5/2, ΔospC/ΔNt10/1 and ΔospC/ΔNt10/2.

Double selection for tio-3 (ppt) and pMSP-TIO (kanamycin) constructs led to the isolation of 19 nineteen transformants containing pMSP1-TIO and 10 containing pMSP2-TIO.

Although transformants with the native NovQ did not produce prenylated polyphenols, modification of its codon usage led to the production of 6-dimethylallylnaringenin and 6-dimethylallylgenistein in transformants following naringenin supplementation.