Sentence examples for transformants isolated from from inspiring English sources

Diagnostic PCR was carried out on 16 hygromycin resistant transformants isolated from each background (HLS1000, HLS1001 and IPO323).

All the transformants were isolated from white colonies on X-gal/isopropyl-beta-D-thio-galatopyranoside agar plates.

Single colony transformants were isolated from minimal medium agar plates containing 2%% maltose and the ability of both genes to restore growth of the EBY.VW.4000 transformant strain in the presence of different monosaccharides was studied.

Through liposome-mediated transformation, both vectors rendered transient antibiotic resistance to Thermotoga cells in liquid media, but no transformants could be isolated from plates.

In order to confirm that the G. graminis LOX gene was successfully transcribed in the A. nidulans GG-LOX transformant mRNA was isolated from both the wild type and the A. nidulans GG-LOX.

The recipient genomic DNA was isolated from transformants obtained under the following co-cultivation conditions: the ratio of bacterial cells to target conidia was 5 × 103 or 1 × 104 on solid IM (Table 3, 48 h) and 50 in liquid IM (Table 5, 48 h).

Plasmids were isolated from transformants using the QIAprep Spin Minprep kit (Qiagen, Hilden, Germany), and inserts were sequenced by a commercial provider.

Fungal DNA was isolated from transformants by standard protocols.

Eight XmnI restriction patterns were observed among the NDM-encoding plasmids isolated from transformants of the isolates from the United States and isolate 0S-506 from Sweden.

Restriction analysis of plasmids isolated from transformants showed 6 similar restriction profiles for Newport isolates (R1 R6).

Yeast genomic DNA was isolated from transformants and PCR-amplified as described previously [ 29].