Your English writing platform
Discover LudwigSuggestions(2)
Exact(1)
In such a case, serotype shifts can indeed be triggered by temperature alterations, but the selection of individual transformants is required.
Similar(59)
For many applications transformants are required to harbor only one copy (per haploid genome) of the transgene to increase the stability of the construct over many generations of subsequent breeding.
These results indicate that ura- selection is required for the transformant to show laccase activity, which is maintained in ura- selective cultured media.
When four codons are mutated, a pool of ~10 E. coli transformants would be required to ensure detection of the rarest combinations.
Due to the inability of the Δ areA mutant to utilise various nitrogen sources, no additional resistance marker was required and transformants were regenerated on media containing nitrate as the sole nitrogen source.
Transformants are fastidious and require longer subculture intervals than wild type A. marginale.
When required, transformants were plated onto solid medium containing 5′-fluoroorotic acid (1 mg/ml) to select for the loss of the URA3 marker.
Stable transformants were obtained by streaking on TrMM plates containing the required selection pressure, for two successive rounds.
Due to rapid and sharp selection, PmR transformants were readily isolated after six days, which is half the time required for previously used markers.
The plant transformation was performed as described previously59, and the transformants were selected for kanamycin resistance on MS medium.
Since the number of original transformants being expressed within each pool was estimated to be 1,000, the identification of an individual antigen requires further testing.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com