The resulting transformants are functional for studying dynamic bacterial processes in living host cells.
Transformants were obtained with an efficiency similar to that obtained with a replicative vector, providing evidence that this ARS element is functional in S. cerevisiae.
To test whether the WRKY45-myc proteins were functional, we tested the WRKY45-dex transformant lines (T2 homozygotes) for blast resistance.
The receptor was functional as demonstrated by the ability of the transformant to grow on iron-deficient media supplemented with hemin, an ability that the unmodified expression strain lacked.
Primary transformants exhibiting complementation of the nof1-1 phenotype in their progenies were obtained, indicating that the chimeric construct was functional.
Transformants were first selected for functional β-glucosidase phenotype followed by screening for organic solvent tolerance in acetonitrile, dimethylformamide, dimethyl sulfoxide, methanol, 2-propanol.
The resulting transformants were screened for a functional phenotype by direct selection on minimal media containing 10 μM GSH.
The randomly mutagenized plasmid was directly transformed in S. cerevisiae met15 Δ hgt1 Δ strain and selected on 10 µg glutathione containing plates, and functional transformants were used to prepare plasmid DNA following a retransformation.
