Sentence examples for transformants in which from inspiring English sources

Normally, 100  μL of the transformation suspension was plated on 9 cm agar plates, except for the big-scale screening experiment of the LC plasmid library on rich agar media with glucose (plating above 100000 transformants) in which 2.1 mL of the transformation suspension was plated on square Corning bioassay plates (24.5 × 24.5 cm).

To understand the function of ystA, a deletion construct was generated that removes the entire transcribed region and transformation of the G526 strain yielded a number of transformants in which the locus had been deleted (as verified by Southern blot hybridization; data not shown).

Because an equal amount of 21-nt and 22-nt miR168 species is observed in 35S2∶MIR168b transformants in which miR168 is predominantly produced by the 35S2∶MIR168b transgene (Figure 3A), the mir168a-2 mutant, which produces miR168 only from the MIR168b locus, was expected to produce an equal amount of 21-nt and 22-nt miR168 species.

Transformants in which spacer #1 was present grew more poorly.

Positive transformants in which the plasmid had been repaired were selected for on SC-Ura media.

Transformants in which PaGlo1 is deleted were selected by growth on phleomycin-supplemented medium.

As shown in Fig. 6A, a large number of MFIBs with strong autofluorescence were formed in the transformant in which Ald4p was overexpressed, whereas the parent Δ ald4 cells did not form any MFIBs.

One of the transformants (GP1241), in which the integrative suicide vector drove the integration of the PP- emm6 fusion into Tn 916, was isolated and analyzed for M6 protein expression.

The transformant strain in which the truncated pfk13 gene of A. niger was expressed performed more efficiently as it was able to grow successfully in glucostat cultures with 277 mM glucose – while the optimum glucose concentration for the parental strain was 55 mM.

However, the transformant ΔpdeH/MPG1-2, in which MPG1 expression level was almost the same as that of the ΔpdeH mutant, could not complement the defects (Figure 4A and 4B).

To further investigate the role of CPBF1 in EhCP-A5 trafficking and processing, we generated an amebic transformant strain in which the CPBF1 gene was repressed by gene silencing (Bracha et al., 2006).