Sentence examples for transformants in tissue from inspiring English sources

G418 resistance allows selection of stable transformants in tissue culture, the luciferase expression cassette allows efficient quantitation of gene expression in tissue culture and in vivo while oriP/EBNA-1 allows stable maintenance as episomes in tissue culture and possibly in vivo.

For this reason, transgenic potatoes are maintained as vegetative clones from the initial selection of the transformant in tissue culture through to commercial release [ 3].

For analysis of starch physicochemical properties, independent primary transformants were multiplied by excising axillary buds of plantlets in tissue culture and regenerating them to whole plants and tubers in subsequent summers.

Five co-transformants obtained from two independent callus lines were identified in tissue culture by PCR using primers specific for the coding regions of the transgenes.

Primary switchgrass transformants and co-transformants were identified by PCR with total nucleic acid extracts from leaves of plants in tissue culture (REDExtract Plant PCR Kit, Sigma-Aldrich, St . Louis MO, USA) using primers specific for the coding regions of the transgenes (Table  3) and the amplification conditions described previously [ 7].

Fig. 3 Amylolytic activity of yeast transformants in plate assays.

Nevertheless, different modes of transgene silencing might affect the screening efficiency of transformants in plant species, which is assumed to cause difficulty in obtaining transformants.

Transformants in which spacer #1 was present grew more poorly.

We were only able to obtain pure, homokaryotic transformants in the case of the ΔhacA transformants (compact growing colonies), whereas propagation of the ΔireA transformants was only possible when substrate mycelium was transferred (data not shown).

A range of methods were attempted with the early experiments (potato and carrot) in order to successfully isolate putative transformants from plant tissue.

Filament elongation was reduced in the transformants, resulting in the heterostyly type of floral architecture.