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Non-transformed Gerbera 'Terra Regina' inflorescences, grown side by side with the transformants in the greenhouse, contained on average about 900 individual flowers.

Fig. 4 Assessment of genetic stability of transformants in the absence of selective pressure.

The constructed vector was stably maintained in the transformants, in the presence or absence of butanol stress.

PCR screening using adaptor primer and VRV (CT 15 as primers identified 104 microsatellite clones from 422 transformants in the libraries.

Figure 2 Correlation between the level of transcription of the aphII gene and the survival rates of different transformants in the presence of 100 μg.ml -1 neomycin.

The transformants in the present study produce the highest total IBE concentration reported for clostridial batch cultures without online IBE removal (24.4 g /L).

All the uidA transformants had the "yellow-in-the-dark" phenotype characteristic of chlL mutants, whereas homoplasmic nifH transformants exhibited a partial "green-in-the-dark" phenotype.

Transformants, in which the kusA locus was disrupted by the DR-flanked amdS marker gene, were screened for growth on acetamide and via Southern blot analysis.

For comparison, induction factors were also calculated from pPsaD-GLuc-derived transformants, in which the luciferase gene is driven by the psaD promoter.

The stable uptake of DNA over generations depends on the molecular factors governing the integration of DNA into the recipient's chromosome, and the environmental and population factors that determine the long-term survival of the transformant in the population.

Positive transformants in which the plasmid had been repaired were selected for on SC-Ura media.