Sentence examples for transformants identified by from inspiring English sources

Transformants, identified by resistance to the antibiotic hygromycin and confirmed through PCR, were recovered at a very low rate of less than.01%.

Primary switchgrass transformants and co-transformants were identified by PCR with total nucleic acid extracts from leaves of plants in tissue culture (REDExtract Plant PCR Kit, Sigma-Aldrich, St . Louis MO, USA) using primers specific for the coding regions of the transgenes (Table  3) and the amplification conditions described previously [ 7].

The recombinant plasmid was transformed into E. coli JM109, and transformants were identified by restriction digest analysis and sequencing.

For yeast infectivity assays, cell extracts were co-transformed with pRS316 into YJW187 spheroplasts; [ PSI+] URA+ transformants were identified by plating the yeast cells in top agar containing synthetic defined medium lacking uracil and adenine (SD-Ura-Ade) and supplemented with 10 mg/ml adenine hemisulfate (Sunrise Science, San Diego, CA).

Arabidopsis transformation was performed by the flora-dip procedure [ 40] and transformants were identified by screening for kanamycin resistance.

Positive transformants were identified by sequencing (Baseclear, Leiden, The Netherlands).

The transformants were identified by PCR, Southern blotting, Western blotting after selecting on resistant media.

Homoplasmic nifH or uidA transformants were identified by Southern blots after selection on minimal medium plates for several generations.

Linearized recombinant plasmid pHILGAG by SalI was electrotransformed into the yeast strain GS115, and the yeast transformants were identified by PCR.

Transformants were identified by selecting for 5-FOA resistance on rich glucose media.

Multiple transformants were identified by screening on plates containing 30 mg l-1 kanamycin.