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The target gene mRNA was reduced to a level exceeding 90% in clones appearing in all three siRNA-introduced transformants; however, in none of the clones was Fc oligosaccharide fucosylation completely abolished.
As expected, increases in final cell concentration (GK1_7: 108%, GK2_16: 116%) and dry cell weight (GK1_7: 103%, GK2_16: 110%) were observed in both transformants; however, in comparison to GK1_7 cultured without glycerol (Table 1), the addition of glycerol did not significantly affect all tested parameters, suggesting that the GK1 gene may not be fully functional.
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Transformation studies were not successful in selecting transformants; however, the method would have only extracted small plasmids.
On August 14, A was significantly greater at elevated CO2 for both WT and transformants, however, there were no detectable differences in photosynthesis between WT and transformants.
In contrast, a low density of A. tumefaciens yielded fewer transformants; however, the T-DNA predominantly existed as a single copy in the recipient genome.
Oosumi et al. [ 4] did not report the occurrence of tetraploidy among their transformants; however, a careful examination of the four transformants that were photographically documented by these authors suggests to us that the transformant depicted in their Figure eight-b [ 4] may be a tetraploid, as suggested by the distinctive leaf morphology that we have shown to be indicative of tetraploidy.
However, in some transformants (e.g., PaGlo2_OEx strains) a further signal is visible, slightly above PaGLO1.
However, in the best primary transformants, seed viability appeared totally impaired and all attempts to overcome the problem were unsuccessful (Reggi et al.2005).2005
However, in contrast to 35S2∶MIR168a transformants that over-accumulated a miR168 species that co-migrated with the endogenous 21-nt miR168 [28], 35S2∶MIR168b transformants over-accumulated equal amounts of 21-nt and 22-nt miR168 species (Figure 3A and data not shown).
However, in pot experiments, only one transformant, Rc4-4, exhibited a much higher ability to control soybean Sclerotinia stem rot.
However, in NER-proficient E. coli, comparable numbers of transformants were obtained from either type of plasmid, regardless of whether the cross-link was reduced or not.
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