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25 transformants having a faster growth rate were picked from YPD plates with G418 at a final concentration of 4 mg/mL.
Transformants having a better growth rate on MD plates were streaked on YPD plates containing different concentrations of G418 (0.5, 1.0, 2.0, 3.0, 4.0 mg/mL).
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In 5 of the 11 transformants, the flower longevities were longer than that of the wild type, indicating that the transformants had a reduced sensitivity to ethylene than the wild type.
When grown in calcareous soil until harvest, the transformants had a 7.9 times higher grain yield than vector controls (Fig. 4b,c; [17]), demonstrating that creating a complete strategy I system in rice by enhancing ferric-chelate reductase activity is extremely effective in improving Fe deficiency tolerance.
Arabidopsis transformants have a significant increase (P = 3.9×10−93) in GUS activity in response to the TNT ligand.
We next examined the structure of the plasmids within transformants grown on –leucine plates by randomly recovering plasmids and digesting them with restriction enzymes and found that >70% of plasmids recovered from the transformants had a desired structure (Table 1).
The E. coli transformants had a β-lactam resistance pattern that corresponded to the expression of a blaKPC-like gene.
Thus for a small increase in protein, transformants had a significantly greater increases in nitrogen use efficiency than WT at elevated [CO2].
Both transformants had a specific enzyme activity of 1.5 mU/mg proteins, which was much higher than the wild-type in which an activity of 0.27 mU/mg protein was measured (Table 4).
Since the transformants have an overall decrease in secondary wall development, the reduction in lignin may simply reflect that.
Each transformant had a single band with different sizes, indicating that the transformants contained a single copy of Sh ble integrated at different locations of the genome.
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CEO of Professional Science Editing for Scientists @ prosciediting.com