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Transformants generated by co-transformation with pHRLucP revealed an up to 22 (± 1 -fold induction (EuHR-8, Figure 6D).

In contrast, luciferase activity was detected in several transformants generated by co-transformation with the plasmid pHRLucP.

Extracts of transformants generated by co-transformation with the plasmid pHsp70A-GLuc showed a significant but mostly moderate luciferase activity when cultivated under standard conditions at 29°C.

For transformants generated by co-transformation with plasmid pHsp70A-GLuc, a size of 31 kDa was calculated for the intact, unprocessed HSP70B/Gluc fusion protein.

Protein extracts of transformants generated by co-transformation with the pHRLucP or pHsp70A-GLuc plasmid were separated by standard SDS-PAGE [ 85] without using thiol reagents.

In extracts of transformants generated by co-transformation with plasmid pHRLucP the luciferase activity increased between 33- and 120-fold compared to algae that were kept at 29°C.

Transformants were generated by co-transformation with the selectable pPmr3 plasmid and one of the three gluc-derived, non-selectable reporter gene plasmids at a time.

Transformants were generated by the biolistic transformation method.

This method was successful in silencing the gene, and the iron uptake of the transformants generated with this construct was reduced by roughly 50%%.

Transformants generated using the CRISPR Cas9 system were screened by colony PCR using the primer pair P_Km01-010 + P_Km01-010-011 (Additional file 1: Table S1).

To eliminate the possibility that Ura+ transformants were generated by telomere addition at both ends of the linear vector, independent Ura+ transformants were analyzed by pulsed-field gel electrophoresis and all were confirmed to have full-length CF products.