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These ranges in activity were similar to those obtained in the initial populations of transformants from which we propagated the sweet orange and citrange plants under investigation [ 33, 35].
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High genotoxicity of the adducts was manifested in the low yield of transformants from the constructs, which was 2 5% in most strains but increased 2 4-fold with SOS.
The index case also harbored a KPC- Ko (CAV1015) (4), from which a second GeneHogs transformant (GH1015) was generated.
The retention of the inserted SIV DNA fragments was verified by restriction analysis and validated transformant cultures were grown at 37°C using larger plates to produce recombinant populations from which gas vesicles would be harvested.
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from which they originated.
DNA was sequenced from the transformants, which confirmed the presence of the suaA23 mutation.
For comparison, induction factors were also calculated from pPsaD-GLuc-derived transformants, in which the luciferase gene is driven by the psaD promoter.
A The relative retrotransposition frequency was calculated in the same way as for Table 2, but data was taken from only the particular transformants in which RNA levels were also measured.
Interactions were assessed by plating several double transformants from "non-selective" media (media that allows for identification of the double transformants) on which growth is possible only if there exists an interaction between the test subjects.
To exemplify this approach which could be extended to shRNAs and to animal cells using viral libraries at low multiplicity-we have used a bank of yeast transformants which differ from each other with regard to single ectopic cDNAs.
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