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Selected transformants from the disruption transformation with reduced growth and from the multicopy transformation with improved growth on starch were used for Southern analysis and demonstrated that multiple disruption and multicopy transformants were obtained (data not shown).
Eight individual transformants from the CX3- and ten from the CX5-suppression rice lines were detected positive for hptII, GUS and CX3 (or CX5) genes (Fig. 1a).
To isolate one albino mutant with a 95% probability out of 5,500 transformants from the approximately 35-Mb genome of A. aculeatus, it was calculated that the T-DNA must disrupt gene the function in a 19-kb region around the T-DNA integration locus (Krysan et al. 1999).
Sequence analysis of the transformants from the gene-replacement experiment confirmed that the INU1 ORF had been replaced by sequences encoding scFv; two of the resulting constructs were designated strains No. 6 (Km02-064) and No.7 (Km02-065) (Figs. 1b and 3b).
Almost all of the transformants from the 3 groups, where the linearized plasmids with inserts were combined, showed leucine synthesizing ability (Figure 2C).
Transformants from the yeast library pools were isolated as single colonies and their plasmids isolated.
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It was discovered that the transformants from mice that succumbed to the infection had lost the RNAi vector, and in the healthy population, the vector was still present.
These ranges in activity were similar to those obtained in the initial populations of transformants from which we propagated the sweet orange and citrange plants under investigation [ 33, 35].
The nature and amounts of Lig-PCR producan can be used as an indication for the expected transformants from ligation mixtures, further supporting the usefulness of this method.
Independent transformants derived from the same reconstitution construct showed consistent fluorescent signal and undiversified phenotypes, including hyphae growth, conidiation and pathogenicity, in M. oryzae.
Likewise, the luciferase activity in transformants differed from the initial values by only approximately ± 6%.
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