Sentence examples for transformants from each from inspiring English sources

To determine whether the I- SceI-mediated targeted integration correlated with the expression of the introduced glucoamylase cassette, we assayed ~40 stable transformants from each transformation group for glucoamylase activity and screened the glucoamylase-positive transformants for their pyrG phenotype.

The result is representative of at least five independent transformants from each treatment.

Ectopic transformants from each wild type were selected and called Fec (Ferrara) and Aec (Ahlburg).

The cellulase production levels of transformants from each construct were assayed on β-glucan plates (see below).

The transformants from each HA mutant library replicate were pooled, cultured in LB supplemented with ampicillin, and mini-prepped to generate the HA codon mutant plasmid libraries.

In this work, we tested the generation of multicopy clones by the PTVA process starting with 55 transformants from each cassette.

To this end, inducibility was determined by measuring luciferase activities in the uninduced and induced states for the four best-expressing co-transformants from each construct (identified among 32 randomly picked clones).

Diagnostic PCR was carried out on 16 hygromycin resistant transformants isolated from each background (HLS1000, HLS1001 and IPO323).

Because almost all cells were able to recover after the temporary G418 selection, we obtained more than 100,000 transformants from one transformation.

Selected transformants from the disruption transformation with reduced growth and from the multicopy transformation with improved growth on starch were used for Southern analysis and demonstrated that multiple disruption and multicopy transformants were obtained (data not shown).

Transformants from five independent transformation experiments (i.e., 33 clones) were tested for their maximal antibiotic resistance.