Sentence examples for transformants for the from inspiring English sources

The recombinant plasmid pCAMBIA1300-hph-PsCT pCAMBIA1300-hph-PsCT pCAMBIA1300-hph-PsCT containingd and transtrongd into T. rexpressionoptimized Agrobacassettemediated transformation, producing 324 positive T. reesei transformants for the twassteps of sconstructed

We further analysed genomic DNA from selected transformants for the integration pattern of the glucoamylase cassette by Southern blot.

Colony PCR was performed using forward and reverse primers flanking the intron insertion sites of the targeted genes to screen transformants for the desired gene insertions.

Colony PCR was performed using forward and reverse primers flanking the intron insertion sites of Ccel_1894 to screen transformants for the desired insertion.

Plasmid transformants for the index case isolates (CAV1016 and CAV1015) were generated by extraction of plasmid DNA and subsequent electroporation into E. coli GeneHogs (Invitrogen, Grand Island, NY).

We used a pair of primers specific to the zeta-globin promoter region to screen PCR transformants for the Tg.AC transgene sequence [ 7].

b The diagram indicates the corresponding co-transformants for the assay in c. c Transformants were streaked on QDO medium supplemented with 40 µg/ml X-α-Gal.

For the ade2 assay, the numbers of Ade+ Ura+ transformants were compared to Ade− Ura+ transformants, while for the leu2 assay Leu+ Ura+ transformants were compared to the number of Leu− Ura+ transformants.

The activity of acetoacetyl-CoA reductase, measured by the oxidation of NADPH, is similar for all transformants except for the negative control (transformed with an empty plasmid).

At least 5 - 10 independent transformants for each of the four constructs were screened for homozygous transgenic plants in the T2 generation based on segregation of the selectable marker gene.

Luciferase activity was determined, in TAP ENEA2 medium, uninduced or after induction with 50 µM Ni or 10 µM TETA, on 24 transformants for each of the five constructs shown in figures 1A and 6A.