Sentence examples for transformants for each from inspiring English sources

Thus 60,000 transformants for each (enzyme:vector) pair were independently obtained after ligation, transformation of competent E. coli cells using standard methodology, and growth on selective LB medium.

Six or more independent transformants for each transgene construct were analyzed to determine the ability of the transgenes to genetically rescue their respective null mutants and all lines for a given construct were found to display the same phenotypes.

Surprisingly, however, and although we screened at least 100 primary transformants for each construct, we were unable to detect an EGFP signal in nonfunctional SUB cSUBmut:EGFP sub-1 plants (Figure 7M P).

Luciferase activity was determined, in TAP ENEA2 medium, uninduced or after induction with 50 µM Ni or 10 µM TETA, on 24 transformants for each of the five constructs shown in figures 1A and 6A.

We analyzed at least 50 independent primary transformants for each construct in wild-type and sub-1 backgrounds and continued with lines that showed detectable root signal for further analysis.

We preserved at least four individual transformants for each strain.

Accumulation of Cbh1 and CelD in the medium was analyzed by SDS-PAGE and one transformant for each enzyme was used for further investigation.

However, we did not observe GUS activity in any of the 30 independent stable transformants produced for each construct, even after prolonged staining (data not shown).

The number of transformants required for each (enzyme:vector) pair to give a 99% confidence level that all sequences of the genome are represented with a mean insert size of 2 kb was 30,000.

The transformants obtained for each of these constructs were purified through single spore cultures, and integration of the expression construct to the cbh1 locus was verified by PCR analysis.

To generate the yeast transformants for YTH analysis, DNAs from each of the 59 generated "bait" pGBKT7-derived constructs were isolated from E.coli, and transformed into yeast strain AH109, resulting in a collection of different yeast transformants that expressed a set of 59 DNA-binding domain (BD) hybrid proteins (Supporting Information Table S2).