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The frequency of transformation was determined by calculating the number of transformants for a given amount of viable cells (5×108 bacteria) with 1 µg DNA of the suicide plasmid (pILL796).

We screened a total of 290 primary Arabidopsis transformants and 97 primary tobacco transformants for a ligand dependent response (Table S1) by following changes both visually and quantitatively.

A large number of transgenic wheat plants were generated and subjected to successive screenings and selection of FHB resistant plants starting with the primary transformants for a period of over four years.

Our results with two independent transformants for each construct show a clear difference between the long and the short acidic domains (Fig.  8): whereas the long acidic domain encoded by ZFY, Zfy2 and Zfy1 activated β-galactosidase production like the acidic domain of Gal4, none of the three short acidic domains activated at all.

The results of the assay are shown in Figure 4, positive transformants for lipoxygenase activity are visible as a brown colony while wild type did not stain.

Utilizing Agrobacterium-mediated transformation, we generated 78 primary transformants for LeTGA1 knockout and 130 primary transformants for SOLly GLB1 knockout.

Single colonies of P. pastoris transformants for each gene were grown in an orbital shaker under induction conditions.

These results were observed in multiple independent transformant lines for a given construct.

From a pool of 75000 transformants for each block of three Cys, we identified multiple mutants that preserved drug resistance and yeast mating activity.

GUS activity was assayed in a minimum of ten primary transformants for each construct.

Surprisingly, however, and although we screened at least 100 primary transformants for each construct, we were unable to detect an EGFP signal in nonfunctional SUB cSUBmut:EGFP sub-1 plants (Figure 7M P).