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The transformants exhibited higher tolerance to NaCl stress compared to untransformed parent plants.
BALB/c mice inoculated with transformants exhibited higher weights, lower histological lesions and lower M. tuberculosis inoculum in the liver, spleen and lungs than control mice challenged with wild-type M. tuberculosis.
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The transformants also exhibited higher photosynthetic productivity.
In vitro assays showed that most transformants exhibited a higher parasitic ability against sclerotia compared to the wild type strain.
Thus, Volvox transformants exhibited an up to 125-fold higher tolerance against paromomycin than the wild-type strains.
Consistent with the results of the Chi67-1 expression and chitinase activity measurement described above, the Rc4-4 transformant exhibited the highest control efficiency against soybean Sclerotinia stem rot (81.4%), which was 31.7 and 28.7% higher than seedlings treated with the wild type strain and carbendazim, respectively (Table 4; Fig. 2b).
However, the transformants exhibited no improved tolerance against desiccation stress.
Resultant transformants exhibited salt-tolerance with dwarfing phenotypes.
All the uidA transformants had the "yellow-in-the-dark" phenotype characteristic of chlL mutants, whereas homoplasmic nifH transformants exhibited a partial "green-in-the-dark" phenotype.
Compared to the wild type, the PYC activity in the pyc transformants increased 56%83%%, whereas pepc transformants exhibited significant PEPC activity (3 6 mU/mg) that was absent in the wild type.
The MerE transformants exhibited no distinctive phenotypes.
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