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However, the transformants exhibited a wide variation in sporulation, ranging from 5.3 to 57.2 × 107 spores plate−1.
In vitro assays showed that most transformants exhibited a higher parasitic ability against sclerotia compared to the wild type strain.
All the uidA transformants had the "yellow-in-the-dark" phenotype characteristic of chlL mutants, whereas homoplasmic nifH transformants exhibited a partial "green-in-the-dark" phenotype.
Fifteen transformants exhibited a MIC of 16 μg/ml, whereas the remaining three transformants exhibited a MIC of 8 μg/ml.
H-ras transformants exhibited a statistically-significant increase (p < 0.05) in foci count, as compared to non-treated NIH3T3 controls (Fig. 5a & c).
During the course of the experiments, we repeatedly noticed that RASSF2A transformants exhibited a round morphology, suggesting that RASSF2A expression leads to the inhibition Ras and, in turn, inhibition of focal adhesion and stress fiber formation and activation of paxillin.
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Transformants exhibited an increase in resistance to paromomycin by up to 333-fold.
Thus, Volvox transformants exhibited an up to 125-fold higher tolerance against paromomycin than the wild-type strains.
However, when a 50X concentration of substrate was used, five of thirteen transformants exhibited GUS staining in a wild-type INO pattern.
GC-MS analyses of DMDS derivatives prepared from the yeast transformants exhibited the characteristic fragment for a double-bond position between C11-C12 at m/z 245 (Fig. 5A).
Compared to the mock transformant, which exhibited 917 CFUs on a YEPD plate, lac3 transformants exhibited less than 40% growth.
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