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The activity of acetoacetyl-CoA reductase, measured by the oxidation of NADPH, is similar for all transformants except for the negative control (transformed with an empty plasmid).
A massive smear of dsRNA was observed with predominant dsRNA bands around the expected sizes for all transformants except for the untransformed wild-type HT115 bacteria.
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Nevertheless, FAME productivity of the transformants was more than 13%% greater than WT on day12 (except for transformant 3 11 under conditions of osmotic stress).
The pattern exhibited by the four wild-type transformants was wild type, except for an additional band corresponding to the pMoCosX vector.
Our method leverages a colorimetric assay to screen for positive transformants and allows for macroscopic visualization of successful integration events.
Moreover, transformants grown in medium 8 and 20 also show improved itaconic acid production compared to the reference, except for transformant GPD 4.3.
The plant transformation was performed as described previously59, and the transformants were selected for kanamycin resistance on MS medium.
Transformants were screened for growth in the absence of uracil.
Transformants were selected for uracil protrophy.
Transformants were selected for Ura+.
Transformants were selected for uracil prototrophy.
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