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Two OCI-expressing potato lines, K52 and K53, were selected for the experiments from a collection of independent transformants established earlier in our laboratory [ 23].
Reverse transcriptase (RT) PCR, immunodetection and surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI TOF MS) analyses were performed to select transgenic potato lines expressing CCII at different levels in leaves, among a collection of independent transformants developed earlier in our laboratory [ 21].
Early in "W.E".
early in the race.
In general, higher transformation frequencies were observed in E. coli O157 H7 9×1022 to 1.25×104 transformants/µg) than in Salmonella (1.2×102 to 1.4×103 transformants/µg).
earlier in the tournament.
The transformants showed enhancement in photosynthesis rate in high temperature conditions.
These mutants, the wild-type D. discoideum strain AX2 and rescued transformants, were cultured in HL5 medium, harvested at the early growth phase and spread onto 1.5% agar plates at appropriate densities.
Transformants varied in expression level.
Transformants silenced in this gene formed conidia constitutively.
Real-time PCR revealed that there were more PeUMPS transcripts in pUT2 transformants than in pUT1 transformants.
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