Sentence examples for transformants each of from inspiring English sources

Three transformants each of YPH499 harboring the target plasmids (in pCP5.1) and YPH500 harboring the Tev-mTALENs were grown in 96-well plates at 30° overnight with shaking in synthetic complete medium lacking tryptophan and uracil (for the YPH499 target strain) or histidine (for the YPH500 Tev-mTALENs strains).

Second, we constructed a yeast open reading frame (ORF) galactose-induced overexpression array by generating a genome-wide set of yeast transformants, each of which carries an individual plasmid-born and sequence-verified ORF derived from the Saccharomyces cerevisiae full-length EXpression-ready (FLEX) collection.

The best transformants of each of the gene combinations were chosen.

Luciferase activity was determined, in TAP ENEA2 medium, uninduced or after induction with 50 µM Ni or 10 µM TETA, on 24 transformants for each of the five constructs shown in figures 1A and 6A.

Transformants containing each of these five ARS plasmids were propagated in AS168 broth without mevinolin selection and passaged every 4 days.

Five individual transformants for each of the Intracellular ZERA-GLA variants and CBHI-GLA secretion strain were grown in replicate shake flask cultures.

It was used in the present studies to generate the three different transformants populations each of which incorporated one of gene segments of interest, tat (150 bp), rev (243 bp) and nef1 (642 bp).

At least 5 - 10 independent transformants for each of the four constructs were screened for homozygous transgenic plants in the T2 generation based on segregation of the selectable marker gene.

Next, over 5000 transformants representing each of the LC and LU promoter libraries (see above) were screened with respect to increased Zeocin tolerance levels relative to GS115 cells harbouring original plasmid pPPE17 under P AOX 1-induced conditions.

In this screening platform, three different yeast pools were interrogated with a single TAG4 array: a homozygous deletion pool, a heterozygous deletion pool, and a pool of genomic library transformants, each treated with a compound of interest.

The array consisted of a set of yeast transformants, each expressing a HCMV ORF as a hybrid protein with the Gal4 DNA binding domain (BD).