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Our results indicated that these constructs were present in all transformants examined and exhibited similar restriction enzyme profiles as those in E. coli, suggesting that there is no significant change or rearrangement of the sequence of the transformants during the screening process.
Our nutrient consumption data are consistent with the faster growth of transformants during the early stages of culture (Fig. 5).
We show that in ambient and elevated [CO2] plots, carbon uptake was enhanced more for transformants during the vegetative phase (i.e. July 31) than when plants were starting to flower (August 15).
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The growth rate of the MstG transformant during the exponential growth phase was comparable in the three different conditions, while in case of the MstH transformant, at the highest glucose concentration the growth rate was reduced.
Selectable marker genes usually confer antibiotic or herbicide resistance for the selection of transformants during plant transformation.
However, plasmids derived from pKO5 are difficult cloning vectors to use because of the limited number of restriction sites available for insertion and the low frequency of recombinant transformants obtained during the cloning procedure.
In a quantitative analysis, the time course of growth and secreted amylase production by JPU and CEN.PK2 transformants during batch culture were evaluated and are shown in Fig. 4.
Whereas 18 out of 27 4m-AGO1 transformants displayed the 4m-AGO1 phenonlye, only five out of 39 4m-AGO1/4m-MIR168a co-transformants and six out of 46 4m-AGO1/4m-MIR168b co-transformants displayed this phenotype.
Total protein was extracted from the transformants expressing the hMLH1variants.
Transformants harboring the Luc constructs were identified by luciferase assays.
Transformants carrying the desaturase ORFs all produced ALA.
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