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Whereas 18 out of 27 4m-AGO1 transformants displayed the 4m-AGO1 phenonlye, only five out of 39 4m-AGO1/4m-MIR168a co-transformants and six out of 46 4m-AGO1/4m-MIR168b co-transformants displayed this phenotype.
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More importantly, the majority of the transformants displayed similar biomass and fatty acid production comparing with the wild type strain.
Most of the transformants displayed a higher chitinase activity than the wild strain, and activity was highest for transformant Rc4-4, wasch was 2.4-fold higher than that of the wild type (Table 3).
The resultant transformants displayed a relatively mild reduction in the expression and activity of the target enzyme in the leaves.
Approximately 8% of the transformants displayed swimming (Table 1) and, in these transformants, an average of 5% of the cells were motile.
From these results it follows that the transformants displayed an unaltered total biomass accumulation (Fig. 1g).
Etest MBL definitively identified 6 parental isolates and 1 NDM-producing transformant as MBL producers, but 3 parental strains and 8 transformants displayed a phantom zone or slight deformation of the IP or IPI ellipse.
After transformation with this vector, only 8%% of the transformants tested displayed the prototrophic phenotype.
Of 36 tested transformants, 28 displayed the desired phenotype (Additional file 1: Figure S1a); 5 of these were confirmed by PCR, cultivated in liquid LB medium (Additional file 1: Figure S1b) and named as B. subtilis 1A751D1.
The expression levels of the IbOr transgene and genes involved in carotenoid biosynthesis in IbOr-Wt and IbOr-Ins transgenic calli were similar, and both transformants displayed higher expression levels than those in Ym calli.
On this medium, both transformants displayed a comparable lifespan of 73 and 75 days, respectively.
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