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Most of the transformants displayed a higher chitinase activity than the wild strain, and activity was highest for transformant Rc4-4, wasch was 2.4-fold higher than that of the wild type (Table 3).

On this medium, both transformants displayed a comparable lifespan of 73 and 75 days, respectively.

The resultant transformants displayed a relatively mild reduction in the expression and activity of the target enzyme in the leaves.

Etest MBL definitively identified 6 parental isolates and 1 NDM-producing transformant as MBL producers, but 3 parental strains and 8 transformants displayed a phantom zone or slight deformation of the IP or IPI ellipse.

From these results it follows that the transformants displayed an unaltered total biomass accumulation (Fig.  1g).

Interestingly, we also observed several transformants displaying an s phenotype.

As these transformants displayed different growth phenotypes, an association between insert size and slow growth seems unlikely.

Approximately 8% of the transformants displayed swimming (Table 1) and, in these transformants, an average of 5% of the cells were motile.

As shown in Fig. 7, pyrG − transformants obtained displayed a more consistent pattern of glucoamylase activity as compared to the expression profile of pyrG+ transformants.

Among them, 30 transformants displayed obvious restoration of seed set, and five lines were then randomly chosen for a more detailed statistical analysis.

Most of ttg1-13 g4HA-TTG1 transformants displayed the seed phenotypes like wild-type plants (Supplementary Fig. 4a, b), implying that the 4HA-TTG1 fusion protein is biologically functional.