Sentence examples for transformants did not from inspiring English sources
Transformants of pUbDetec16-GAP1 emitted fluorescence, while the pUbDetec16-GAP1K9R,K16R,K76R transformants did not.
Most transformants did not exhibit the DNA structure expected of integration by classical double recombination.
Despite the high level of expression, even higher than some overwintering fish with natural levels of endogenous AFP, the transformants did not display any cold shock resistance compared to controls or low AFP expressing lines.
We again investigated the effects of a higher concentration of A. tumefaciens cells and conidia in liquid IM with the same ratio of A. tumefaciens cells to conidia (50 1), but the number of transformants did not increase in a dose-dependent manner.
Plants with only two components, such as PC transformants and PBcpl1 transformants, did not produce anthocyanins more than control plants (Table 1 and data not shown).
Despite intensive screening, we only got a few mutants (an independent screening of additional 20 000 transformants did not allow to isolate new toxic mutants, data not shown).
The overwhelming majority of the proximal mutations in the AID* transformants do not occur as isolated mutational pairs but, rather, are found in clusters.
Plasmid DNA isolated from C. hydrothermalis was able to transform C. bescii JWCB005 (ΔpyrFA) and C. bescii JWCB018 (ΔpyrFAΔcbeI), however total DNA isolated from the C. bescii transformant did not transform C. hydrothermalis.
Laccase production by the L 61 co-transformant did not appear to decrease even after 5 cycles of subculturing on hygromycin-free semisolid medium (Fig. 4; Additional file 1: Fig. S6).
The transposon donor plasmids we used had an R6K-γ origin of replication requiring specific host factors for replication [29], so that co-transformants do not complicate analysis.
Two 14-mer left and right probes were used to select phagemids containing the correct insert, and transformants that did not hybridize with both the left and right probes were omitted.
