Sentence examples for transformants described in from inspiring English sources

Here it is noteworthy to mention that the copper-depletion mutant grisea in which ∼80% of the electrons in the electron transport chain flow via the AOX [6] displays an increase in lifespan of only ∼60% [1], [2] in comparison to about 100% of the newly constructed transformants described in this paper.

Mean expression values with S.E.M.s were determined from four transformants as described in Figure 3B.

Transformants are described in Tables 1, 2, 3 and 4. Pneumococci were grown in brain heart infusion broth (BHI, Difco) supplemented with 0.5% yeast extract, or in blood agar containing 5% defibrinated sheep's blood.

Emasculated wild-type pistils were pollinated with pollen from the +/gcs1 transformants described above.

A homokaryotic P tcu-1hpt-1 strain was isolated from transformant #6 as described in the Material and Methods.

The AHLs production profiles of these two transformants were analyzed as described in the bioautography analysis section.

A thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) was performed to obtain DNA sequences flanking the T-DNA insertions in the fungal transformants, following the methods described in Liu et al. (1995) and Sessions et al. (2002) with minor modifications, as summarized in Table 2.

Mixed-genotype transformant colonies have been described in other intron-based gene disruption studies [ 24].

The T2 progeny from these transformants were used in the experiments described in the text.

To determine the presence of cphA mRNA in the transformants, liquid cultures were grown as described in the previous section.

To determine and characterize J. curcas cDNA gene sequences that conferred enhanced salinity tolerance at 750 mM NaCl to yeast transformants plasmid rescue was performed as described in Bates [ 22].