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DNA we received from the ORFeome project is purified from a pool of transformants derived from ligation and transformation of their original RT-PCR, thereby allowing the isolation of internal splice variants from the mix.
Independent transformants derived from the same reconstitution construct showed consistent fluorescent signal and undiversified phenotypes, including hyphae growth, conidiation and pathogenicity, in M. oryzae.
Transgenic potato lines expressing CCII were selected from a collection of independent transformants derived from Solanum tuberosum plantlets, cv.
Low transgene copy numbers have recently been reported in transformants derived from embryogenic cultures in several trees [ 21, 23, 38].
Several transformants derived from the cDNA library of V. longisporum in the S. cerevisiae ∆ARO2 mutant strain were obtained on SC medium lacking the three aromatic amino acids.
Dictyostelium Ax2 and DH1 and transformants derived from these strains were grown axenically in HL-5 media in shaken suspension (180 rpm) at 22 °C.
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The first method recovers transformants efficiently and requires fewer plates, but the second method ensures that all transformants derive from independent transformation events.
A Ha-ras transformant '7-4', derived from mouse NIH/3T3 fibroblasts, was used to study the relationship between overexpression of activated Ha-ras and cell apoptosis.
Using the replica-printing based functional screen, 345 candidate yeast transformants expressing cDNA (derived from J. curcas roots) were isolated from a pool of 20,000 yeast transformants.
All clones contained the same 9-bp duplication (ATTTTATGG), indicating that the Rmontanensis700 transformant population was derived from a single transposon integration event.
By only scraping non-overlapping regions from the transformation plate, we ensured that FOAR colonies from different streaks derived from independent transformants.
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CEO of Professional Science Editing for Scientists @ prosciediting.com