Sentence examples for transformants could not from inspiring English sources

Transformants could not be recovered for ΔNCU00434 (ptc-1) and mutants for NCU05049 (dsp-5) were not available (Table 1).

However, prototrophic transformants could not be selected because of the background growth of strain M4 on MA5 minimal agar plates even when M4 cells grown in MA5 medium supplemented with uracil were washed extensively before plating (data not shown).

Among 17 tested HygR transformants, seven could not differentiate Secteurs.

Except for the different number of obtained transformants, we couldn't see any principal differences between transformants generated with pNitLucX and pPmr3 or with pNitLucA alone.

From the transformants obtained, those that could not grow on plates containing galactose (for induction of αSyn expression) and 5-FOA (for loss of the cDNA plasmid) were selected for an αSyn toxicity test.

No fluorescence was observed in any tissues of any transformants assayed, and AML1 GFP transcripts could not be detected via RT-PCR performed on cDNA transcribed from RNA extracted from bulk shoot tissue (data not shown).

As this band hybridized with psec3-s1 and the hph hygromycin resistance marker (data not shown), these transformants could be interpreted as bearing a co-integration of pBC-Hygro and pSecs1 at an ectopic position.

We did obtain a GFP- TDegF CDC28 integrated strain with the CDC19 -500 promoter but could not obtain any transformants with the TEV plasmid, probably because the expression of Cdc28 from the CDC19 -500 promoter was already close to the lower limit of Cdc28, even in the absence of TEV protease.

Then, to find out if S. sobrinus DSM 20742 is able to enter genetic competence state at all, we tried to transform S. sobrinus with plasmids replicative in other Streptococcus spp. like pDL278 (Spr, pAT18 Emr, with suicide vector pFW5 Spr in both circular and linearized forms but could not obtain the transformants.

Further studies demonstrated that by reducing the concentration of DNA used for transformation, the number of such double transformants could be reduced and also result in higher overall transformation efficiency (data not shown).

The native spr0479 gene copy could not be removed (<10 <span class="lh">transformants ml−1) unless 10 μM ZnCl2 was added to the medium (3.8 ± 0.8 × 10 transformants ml−1, n = 2).