Sentence examples for transformants could be from inspiring English sources
Further studies demonstrated that by reducing the concentration of DNA used for transformation, the number of such double transformants could be reduced and also result in higher overall transformation efficiency (data not shown).
In our experimental design the intein was initially transformed into a diploid strain so that transformants could be recovered even if the integration of the intein impaired the expression or activity of calmodulin.
Moreover, the targeted integration transformants could be specifically selected by adding 5-fluoroorotic acid (5′FOA) in the transformation medium.
In both cases lipoxygenase production in the transformants could be identified.
Our data indicate that the tagged genes in almost all of the transformants could be recovered by a combination of TAIL-PCR and inverse PCR, which satisfies the requirement for a successful gene tagging protocol.
Therefore, we expected that transformants could be obtained from a high density of infected germlings on SM plates after A. tumefaciens cells had successfully infected the fungus at the best ratio and concentration, followed by filtration to concentrate the infected germlings and subsequent transfer to SM plates.
As to unmethylated plasmid, no transformant could be found on erythromycin plate.
Plasmid location of the blaKPC genes was confirmed by electroporation of these plasmids into E. coli DH10B, but no transformant could be obtained for K. pneumoniae 2020532.
So, 2.5 times improvement of glutathione production in MOXp-GSH2 transformant could be explained by insertion of cassette into some regulatory locus important for GSH biosynthesis or under unknown promoter.
Using this methodology, the yNhANT2 knock-in transformant clones could be isolated, and these yeast grew on YPEG.
Although successful E. coli transformants could always be obtained for subsequent transcriptional analyses, the in-depth mechanisms might be an interesting question subject to further investigation.
