Sentence examples for transformants containing a from inspiring English sources
Protoplasts of the P. anserina wild-type (s) were transformed with the verified pSM4-PMt-eGfp plasmid, and hygromycin B resistant transformants containing a complete genomic integration of the plasmid were selected by Southern blot analysis (data not shown).
We used two pairs of primers to identify transformants containing a human HPRT sequence.
Y187 yeast cells pre-transformed with AD vectors were mated with yeast AH109 transformants containing an empty DB vector and plated on nutritionally selective medium deficient in tryptophan, leucine, adenine, and histidine.
At least four transformants containing an insert were selected and sequenced in both directions using an ABI 3700 capillary Sequencer with the ABI BigDye terminator chemistry (The PE Corporation, Foster City, CA).
Transformants containing an integrated vector were then treated with 5-FOA to select for excision of the plasmid sequences leaving the EI expression cassette in the chromosome.
Sterol profile analysis demonstrated that shoots of the OsLIC transformants contain a higher level of both typical and atypical sterols, which further supports the above hypothesis.
The expression analysis showed that transformants contained a single copy chromosomal integration of optimized pel168 gene.
Each transformant had a single band with different sizes, indicating that the transformants contained a single copy of Sh ble integrated at different locations of the genome.
After 6 days of culture, SDS-PAGE showed that the media from the culture of the transformants contained a specific protein abundantly (Fig. 3a) and that they had a significant protease activity for Z-Glu-Tyr, whereas that from the culture of the host strain showed scarcely detectable activity.
Here, a clone is defined as a PAO2003 transformant containing a distinct region of genomic DNA within its plasmid based insert DNA.
Transformant plasmid DNA was evaluated by electrophoresis, and a representative transformant containing a single NDM-encoding plasmid was chosen for further study (designated by TF suffix).
