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In non-legume the symbiotic-like responses were observed only in nim1/npr1 mutants, or in NahG transformants, both of which suppress the SA signaling (Fig. 1).
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After overnight incubation at 30 °C transformants of both constructs formed strong green halos.
The best transformants of each of the gene combinations were chosen.
Following treatment with LiAc and DDT and electrotransformation, vector stability was examined in transformants of both tested strains containing pTRKH3.
These results indicated that the primary transformants of both deletion approaches were heterokaryons, containing nuclei with the genotype hacA/pyrG − (ireA/pyrG − ) and nuclei with the genotype ΔhacA/pyrG + (ΔireA/pyrG + ).
Arginine auxotrophs were found in one of 32 transformants of pyrG13 (targeting frequency of 3%) and in 29 of 31 transformants of MR12 (94%).
Transformation efficiencies were expressed as the number of transformants per micrograms of plasmid DNA.
Transformation efficiency (number of transformants/µg of plasmid) for low, medium and large sized amplification products.
The yield of transformants from both lesion-containing constructs was approximately 1% in this strain, either with or without prior UV irradiation of the host.
Transformants of the AX2-214 strain of D. discoideum were selected using 10 μg/ml blasticidin.
Molecular analysis of transformants Genomic DNA of the transformants was extracted using a phenol chloroform method (Seiboth et al. 2002).
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