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We note that ∼1% corresponds only to the frequency of strong [ PSI+] transformants and therefore represents a conservative estimate of E. coli cell extract infectivity; we did not attempt to quantify weak [ PSI+] transformants because they are difficult to distinguish from [ psi−] transformants on the medium utilized to isolate transformants.
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These genes had been amplified in original transformants, but they were completely eliminated by BA.
Both genes are dominant because they add new, selectable features to transformants, and therefore do not require strains with particular auxotrophic defects as recipients.
"Because they vote".
Because they own guns".
First because they must.
"Because they love it".
Because they are growing.
Because they are entrenched.
Because they want something.
Because they make sense.
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