Sentence examples for transformants based on from inspiring English sources

The idea was to select transformants based on their ability to grow on media requiring a functional mitochondrial respiratory system by providing sufficient adenine nucleotide transport activity to support growth of yeast on nonfermentable carbon sources as described previously [10].

Positive transformants, based on white/blue screening, were picked up and grown at 37°C in 3 ml of liquid LB medium supplemented with ampicillin (50 μg mL-1).

Positive transformants, based on blue/white screening, were picked and arrayed in a 384-well plate containing LB medium (Sigma-Aldrich) supplemented with ampicillin (50 μg mL-1) and glycerol (10% v/v).

MHC-Gal4>UAS-INSR Luc 6, MHC-Gal4>UAS-INSR Luc 6, and MHC-Gal4>UAS-cTNT Luc 87 MHC-Gal4>UAS-cTNT Luc 8 MHC-Gal4>UAS-cTNT Luc 8 on the identificandon of the widest window for luMHC-Gal4>UAS-TnnT3 Luc 7e MHC-Gal4>UAS-TnnT3 Luc 7 luciferase quantification were and without chosenpression induction (iCTG480 flies) (Fig. 2as.

Initially detected in leukemia (Bonnet and Dick 1997), CSCs have now been identified in other tumors and cell transformants based on surface markers, phenotypic traits indicative of malignancy (such as xenograft tumor formation), and in vitro characteristics including matrix metalloproteinase (MMP) secretion, colony formation, and formation of nonadherent spheroids.

Eight strains were selected for each transformant based on the size of the transparent circle in fermentation.

Complemented lines of sta6 (BafJ5) mutant (BafJ5C2, 3, 6, 7, 8, 9, 16, 18, 20) were generated by complementation with plasmid pSL18-STA6 and transformants selected based on paromomycine resistance.

After transformation, the transformants were screened based on the kanamycin resistance marker, and the gfo gene in the selected transformants was confirmed by PCR using primers GFOR-int-5' and GFOR-int-3' synthesized based on an internal fragment of the gfo gene in Z. mobilis, with genomic DNA isolated from the parental strain and transformants as template.

The resulting ligations were introduced into E. coli EC101 (Table  4) by electrotransformation, and transformants were selected based on kanamycin and either ampicillin (pWSK29-based), erythromycin pNZ8048-Em-based pNZ8048-Em-based pNZ8048-Em-basedpNZ44-based).

The transformants were spread on SPASC medium for selection, and several transformants were selected, based on colony size.

After transformation of the Δ xyr1 strain, several independent transformants were isolated based on their recovered ability to form hydrolytic halos on Avicel plate (data not shown).