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Loss of flagella is independent of addition of Ni at 25 μM, since it is observed also in the non-induced transformants at high cell densities.
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The growth rate of the MstG transformant during the exponential growth phase was comparable in the three different conditions, while in case of the MstH transformant, at the highest glucose concentration the growth rate was reduced.
As shown in Figure 3A, the transformants could grow at highest stringency level (–Leu –Trp –His –Ade +α X Gal plates) suggesting the activation of all the reporter genes to the same extent as was observed with p53 and TAg interaction.
We evaluated these transformants for growth at high-salt stress of 750 mM or higher concentration.
Unlike pSY6catP, pHT3 transformed at a high efficiency of 1.8 × 10 transformants μg-1 DNA, which is comparable to pMTL85141ermB.
The transformation system in F. oxysporum was offered by new applications of gene replacement events which were observed at high frequency (in 50%% of the transformants).
On average, electron transport rate (ETR) was significantly higher for transformants at elevated [CO2] (simple effect test; F1,12 = 8.43 p < 0.05).
Twenty to fifty transformants selected at 30°C were then toothpicked on Pm plates and grown at high or low temperature.
The transformants had high FAME productivity under all tested culture conditions, especially at day 8 (Fig. 4b), in parallel with their high DCW.
As expected from the growth pattern, specific growth rate of transformants at the initial period was significantly higher than the rest of the period.
Transformation efficiencies were significantly higher (up to 1 × 103 transformants/μg of DNA for 9.8 kb plasmid pGB301) using late stationary phase cells (OD600 = 1.2) at high cell concentrations (5 × 1010 CFU/ml) when subjected to high voltage electric pulses.
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