The results again showed that the Vsp-transformants associated with HBMEC much more than the wild type (Figure 2B).
However, the higher expression of NsbHLH2 in the transformants was associated with a fast specific growth rate during the early growth phase (first 4 days), resulting in an increased DCW and FAME productivity, especially at day 8.
Conclusively, the improved growth in the transformants can be associated with the enhanced nutrient uptake.
Studies have reported higher levels of recombinant protein in reproductive organs of storage protein-depleted mutants [ 27, 28] or transformants [ 29– 32], presumably associated with increased energy and/or amino acid resources in planta.
The quantitative information described herein should increase awareness of this issue, and the rapid sequencing approach developed for these studies should be widely useful for identifying multiple vector transformants and avoiding complications associated with cells that have acquired more than one unique plasmid.
First, the observed that the association preferentially occurs with endothelial cells as Vsp1-expressing B. burgdorferi transformants like their Bt1 counterpart, associated with HBMEC much more than with fibroblasts or arachnoidal human cells (Figure 1C).
In addition, polysome analysis of the transformants showed less 5S rRNA and rbcL mRNA associated with ribosomes.
Unlike what happened in E. coli where the majority of the activity was associated with cell suspensions, the Thermotoga transformants had about half of the endoglucanase activity found in supernatants).
PCR-based replicon typing was applied to type the resistance plasmids carried by 26 Escherichia coli transconjugants or transformants obtained from epidemiologically unrelated clinical isolates of Enterobacteriaceae associated with community- or hospital-acquired infections in the United States or southern Europe Italy and GreeceEurope Italy and Greece
Rather, the gpt transformants contain one to five copies of the transfecting DNA associated with, and most probably integrated into, cellular DNA sequences.
