Sentence examples for transformants as previously from inspiring English sources

GUS staining was performed on stable transformants as previously described [12].

This co-transformed fragment is coding for a ribosomal protein S12 variant (Lys57Thr) conferring resistance to streptomycin that was used as a surrogate marker for the selection of transformants as previously described [ 35].

Conjugation experiments of index case blaKPC plasmids were performed via mating of E. coli J53 Rifr strain to the clinical isolates CAV1016 and CAV1015 as well as the GeneHogs transformants, as previously described (42).

Procedures for S. gordonii transformation, screening and genetic analysis of transformants were as previously described [ 33].

Marked deletion mutants were obtained by growing the gentamicin-resistant transformants as described previously [ 22].

LacZ expression levels were assayed in quadruplicate on independent transformants as described previously (Rupp, 2002; Nicholls et al., 2009).

Transformants were selected from LB/ampicillin plates, and plasmids were isolated from 2 mL cultures of transformants as described previously (4).

Reporter constructs were transformed into JRY103 and JRY118 and the β-galactosidase activity was measured on three independent transformants, as described previously [ 14].

Mutagenic PCR products were transformed into Y270 as previously described [ 65], and G418-resistant transformants selected for on YPAD containing 200 μg/ml G418 (GIBCO).

DNA transformation was performed as previously described [ 24, 56, 58], and transformants were selected on MH agar plates containing both kanamycin (10 μg/mL) and chloramphenicol (20 μg/mL).

Susceptibility testing showed that both XM1570 and the J53 transformants were susceptible to amikacin as previously observed in A. lwoffii, which was suggested to be a result of disruption in the promoter sequence of aphA6 [ 20, 24].