Sentence examples for transformants as a from inspiring English sources

These recoded synthetic drug-resistant genes could be a useful tool for selecting mitochondrial genetic transformants as a precise technology for mitochondrial transformation is developed.

For it, a PCR reaction was performed using genomic DNA of transformants as a template and gluc-specific oligonucleotides.

After this period, PCR using genomic DNA of these transformants as a template still yielded the expected 422 bp fragment of the aphVIII gene (data not shown).

The presence of the expression cassette in the stable transformants was examined by diagnostic PCR using the primers Ko183/Ko190 and the genomic DNA of stable transformants as a template.

This plasmid was stably maintained in the 01-B526 trassformants, as were the native plasmids, but could be easily cured by removing the selection conditions.

This colour change on the transformants served as a quick visual indication that mCherry was displayed on the yeast cell surface.

Subsequent to transformation process and selecting transformants (as single colonies on LB broth containing ampicillin, as an antibiotic resistance marker), the plasmid was extracted from transformants E. coli and treated with double digesting using BamHI and NdeI to confirm the successful transformation.

The basic idea is to saturate the screening to obtain as many transformants as possible, and to avoid transformation of a single yeast cell with multiple library plasmids, which can seriously complicate subsequent analysis, the lowest amount of DNA needs to be used.

This co-transformed fragment is coding for a ribosomal protein S12 variant (Lys57Thr) conferring resistance to streptomycin that was used as a surrogate marker for the selection of transformants as previously described [ 35].

To disrupt either CCTα or CCTδ genes in the MIC, we introduced neo2 cassette-interrupted targeting fragments into early mating cells using a biolistic gun and produced heterozygous transformants as described [62], [63].

Initially we introduced the integrase expression plasmids into DH5α E. coli K12 strains containing a cognate reporter plasmid and scored the transformants as white or light blue, indicative of recombination, or blue, indicative of no recombination.