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Transformants are selected that replace the marker genes with the mutated oligonucleotide.
Transformants are selected by virtue of their resistance to the drug glufosinate upon recombination of the bar gene (Avalos et al. 1989).
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Transformants were selected on selective minimal medium as described above.
The resulting verified plasmid was transformed into G27 and transformants were selected on Kan 25.
Transposon-mutagenized plasmids were transformed into DH5α and transformants were selected on LB + Amp + Kan plates.
The transformants were selected on a minimal selective medium.
(2) PCR-fused products were directly used to transform B. subtilis, and Zeor transformants were selected.
DY8430 was transformed with pAW8 and Leu+ transformants were selected.
The transformants were selected by 5-FOA counter selection.
The construct was transformed into tpx mutant by electroporation and gentamicin resistant transformants were selected.
HWH11 strain was transformed by YEp352- ALD4 by lithium acetate methods and URA3 transformants were selected.
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