Sentence examples for transformants are often from inspiring English sources

Since S. macrospora primary transformants are often heterokaryotic, we isolated single spore isolates to segregate the wild-type and mutant alleles of cas genes in a Δku70 background.

Because N. crassa is multinucleate, primary transformants are often heterokaryons, with both mutant and wild-type nuclei (Dev and Maheshwari 2002; Paietta and Marzluf 1985; Park et al. 2011a).

The plant transformation was performed as described previously59, and the transformants were selected for kanamycin resistance on MS medium.

The plasmid was transformed into E. coli strain BL21 DE3) RIL and the transformants were cultured at 37 °C in LB medium.

Transformants were selected on kanamycin-containing LB agar plates.

The transformants were selected on the basis of green fluorescence, total genomic DNA (gDNA) was extracted from these fluorescent transformants.

The transformants were cultured and selected.

Transformants were selected on medium lacking leucine at 32 °C to obtain single colonies.

The plasmid complement of transformants was verified by PCR and double restriction digestion.

Selection for double transformants was performed on –Trp Leu SD media.

The transformants were grown at 37 °C until the culture reached an OD600 = 0.6 in LB medium.