Sentence examples for transformants are listed from inspiring English sources

The primers used for the amplification of the cassettes and for the analysis of the transformants are listed in Additional file 1: Table S6.

The strains and plasmids used in this study are listed in Additional file 4. All the Y. lipolytica transformants derived from the genetic background of the W29 wild-type strain and of the derivative auxotrophic strain PO1d (Ura−Leu−) [ 28].

Oligonucleotides used for plasmid construction are listed in the Figure 4 source data 2. Fungal transformation was performed as described previously (Gressler et al., 2011) and transformants were checked by diagnostic PCR and Southern blot analyses (see Figure 4 figure supplement 4).

Positive transformants are visualized as brown dots in the plate.

Transformants were verified via a PCR screen for wild-type hdrABC, the Δ hdrABC operon deletion, and Ptet hdrABC* (in pJC1) using primers listed in Table S1 in the supplemental material.

Cell transformants were pooled and an aliquot was plated to determine the total number of transformants.

Transformants were selected on SD-URA plates.

The transformants were cultured and selected.

Transformants were screened for growth in the absence of uracil.

Transformants were selected on plates with ampicillin and S-gal.

The plates with potential transformants were incubated at 30°C for at least 3 days until transformants appeared.