Sentence examples for transformants and to from inspiring English sources

This method makes it possible to easily characterise the transformants and to correlate expression levels with gene dosage which is valuable when trying to find the P. pastoris clones with optimal characteristics for heterologous protein expression.

Cells were pre-grown in YEPD (1% yeast extract, 2% peptone, and 2% glucose) for transformations, and in YNB (Bacto yeast nitrogen base without amino acids) + 2% glucose supplemented with 30 µg/ml leucine and 30 µg/ml lysine to select for transformants and to produce gadusol.

These results are consistent with previous observations of low frequency stable integration by non-homologous recombination and deletion on the ends of the inserted DNA [ 20, 21, 25], underscoring the need to increase the proportion of stable transformants and to prevent deletion of barcodes.

Total DNA, genomic plus recombinant pRG3-AMA1 plasmids, was extracted from 50 mM sodium acetate tolerant transformants and used to transform Escherichia coli.

Mean expression values were determined from four independent transformants and normalized to the value obtained for the WT strain, and the normalized means and S.E.M.s are reported.

Dilutions were performed as needed in 1× TBase supplemented with 1 mM MgSO4 and cells were plated to LB+spectynomycin plates to select transformants and DSM plates to determine cfu.

We selected 46 independent transformants and allowed each to self-fertilize to generate plants homozygous for the reporter.

After one hour at 37°C, remove an aliquot of the culture for determination of the total number of transformants and add ampicillin to the culture to a final concentration of 50 μg/ml.

Aliquots were taken after transformation to determine the total number of transformants, and after freezing, to determine the titer.

The resulting primary transformants accumulated less CAD RNA transcript and protein than control transformants and were demonstrated to be stably transformed with between 1 and 5 copies of the T-DNA.

For each reporter strain, the basal luciferase activity was measured from pRS314- or pRS315-transformants and used to calculate the fold of induction due to the expression of each p53 family member.