Sentence examples for transformants and selection from inspiring English sources
Co-cultivation period, type of explants, a gfp gene as reporter marker to allow early stage selection of transformants and selection regime were critical factors for successful transformation of Impatiens.
Deletion of this gene results in uracil auxotrophy and resistance to 5-fluoroorotic acid (5-FOA), allowing prototrophic selection of transformants and counter-selection of the wild-type allele [ 22].
A β-glucan plate assay was used for preliminary evaluation of enzyme production by the transformants and for selection of representative clones from the transformation for further analysis.
Constructs were made in E. coli and subsequently introduced in Nme using natural transformation competence and selection of transformants with erythromycin, kanamycin and tetracycline, as the concentrations listed above.
This was digested with EcoRI and SpeI and combined in a 100 1 molar ratio with EcoRI- and SpeI-linearized pBAV1K2 p. Ligation, transformation, transformant selection, and PCR screening were performed as described above.
The constructed plasmids required at least two types of marker genes: one for the selection of yeast transformants and one for the selection of a- or α-type derivative cells.
The resulting 4.3 kb pHimar3 plasmid (containing the HimarFT transposon, the transposase gene, the neomycin phosphotransferase gene under the control of Francisella groEL promoter and the E. coli-compatible origin of replication oriR6K) was transformed into E.coli DH5αλ pir, and selection of transformants was performed on LB agar containing 50 µg ml−1 of kanamycin.
Transformation experiments will make use of the leuphenotype and selection of transformants using LEU2 complementation.
P. pastoris GS115 was transformed with BglII-linearized plasmids by electroporation, and selection of His + transformants and geneticin resistant transformants was done on minimal dextrose medium (MD, Invitrogen BV) and YPD-G418 medium (Invitrogen BV), respectively.
Electroporation and selection of transformants were carried out by using MD and G418.
Synthetic media (SD) lacking specific nutrient supplement were utilized for genotype verification and selection of transformants [71].
